ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological behavior and proper management of myoepithelial carcinomas of salivary glands.</p><p><b>METHODS</b>Twenty-seven cases of myoepithelial carcinoma of salivary glands were retrospectively studied and their detailed clinical and follow-up data were presented.</p><p><b>RESULTS</b>The subjects consisted of 17 men and 10 women aged 16 to 73 years (mean age: 51 years). The parotid gland was the most common site (n = 14) of cancer. Clinical features included extensive local growth, invasion of the surrounding tissues, infrequent cervical lymph node metastasis but high rates of distant metastasis, frequent/multiple recurrences and poor prognosis.</p><p><b>CONCLUSIONS</b>Myoepithelial carcinomas of the salivary gland should be classified as high-grade malignancies. Early and radical surgery with close follow-up are essential for achieving favorable outcomes. Radiotherapy appears to be non-sensitive and elective neck dissection is generally unnecessary.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Myoepithelioma , Mortality , Therapeutics , Prognosis , Retrospective Studies , Salivary Gland Neoplasms , Mortality , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinicopathological features of mucinous adenocarcinoma of salivary glands.</p><p><b>METHODS</b>The clinical manifestations and histopathological characteristics of 6 cases of mucinous adenocarcinoma of salivary gland were studied by retrospective and routine histopathology and immunohistochemistry.</p><p><b>RESULTS</b>Four mucinous adenocarcinoma occurred in palate and 2 in mouth floor. Average age of patients was 60 years (48 - 70) and males were affected more often than females (4:2). Pathologically, the tumor grew with infiltration of surrounding tissues. The tumor consisted of unitary mucinous cells and mucin pool was obvious. The cell pleomorphism and nuclear mitosis were often seen. Some tumors showed acinus-like structure. Tumor cells often formed incomplete duct-like structure and small clusters floating in mucinous pool. There were intracellular mucin and signet ring cells in the tumor. Tumor cells showed positive reaction to PAS, Alcin blue, and some cytokeratin staining.</p><p><b>CONCLUSIONS</b>Mucinous adenocarcinoma of salivary gland is a rare malignant tumor which mainly affects palate and mouth floor of older patients. The tumor may originate from acinic cell of mucous acinus or multi-potential cell of salivary gland.</p>
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Adenocarcinoma, Mucinous , Metabolism , Pathology , Immunohistochemistry , Keratins , Salivary Gland Neoplasms , Metabolism , PathologyABSTRACT
<p><b>OBJECTIVE</b>To evaluate the reliability of Bio-Oss particles as bone grafts in combination with membrane-technique.</p><p><b>METHODS</b>6 small biopsy specimens were retrieved without causing iatrogenic damage in 6 patients (implant sites). The Donath's hard tissue section and micro grinding technique was adopted, and Leval Laczko staining method was used.</p><p><b>RESULTS</b>The histological results showed Bio-Oss particles were easily distinguished from the newly formed bone, but new bone was formed among different particles of Bio-Oss, no connective tissue was found. Intimate contact between newly formed bone and particles of the graft was present.</p><p><b>CONCLUSIONS</b>Bio-Oss appears to be highly biocompatible and osteoconductive. It can be used success fully as a bone substitute in combination with guided bone regeneration membrane technique.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Bone Regeneration , Bone Substitutes , Therapeutic Uses , Bone Transplantation , Guided Tissue Regeneration , Methods , Membranes, Artificial , Minerals , Therapeutic Uses , Oral Surgical Procedures , Methods , Reproducibility of Results , Time FactorsABSTRACT
Objective:To prepare specific antibody against the pre mRNA splicing protein Tra2? in human fetal tissues.Methods:A specific antigen fragment of human Tra2? was cloned into the pGEX 3x expression vector to express a recombinant GST Tra2? fusion protein.The fusion protein was expressed,purified and used as an antigen to immunize the New Zealand rabbit,and the polyclonal antibody to Tra2? was obtained.Results:The antibody was successfully used to detect Tra2? protein in human fetal cerebrum with Western blot and immunohistochemistry analysis.Conclusion:This prepard antibody has good specificity and can be used to detect the Tra2? protein in the human fetal tissues.
ABSTRACT
OBJECTIVE: To study the effects of occlusal trauma on the ultra-structure of synovial membrane and articular cartilage in rabbit's temporomandibular joints (TMJ). METHODS: TMJs from six rabbits with occlusal trauma and three control rabbits were studied by transmission electron microscopy. RESULTS: Degenerative changes in synovial membrane and articular cartilage of TMJ were induced following occlusal trauma. The structure of the articular surface was damaged, and chondrocytes in cartilage showed signs of degeneration. The synovial lining cells contained dense accumulations of vimentin intermediate filaments (IFs), which were especially prevalent in the cellular processes as well as paranuclearly. Microvilli on the synovial cell membrane were commonly seen. The "vermiform bodies" in the deeper interstitium of the synovial tissue were also found. Our findings of the punctate adherens between synovial lining cells were described in detail. CONCLUSIONS: The occlusal trauma is really a factor inducing degenerative changes of the TMJ.